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KMID : 0624620110440120787
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BMB Reports
2011 Volume.44 No. 12 p.787 ~ p.792
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Effect of silk fibroin peptide derived from silkworm Bombyx mori on the anti-inflammatory effect of Tat-SOD in a mice edema model
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Kim Dae-Won
Hwang Hyun-Sook
Kim Duk-Soo
Sheen Seung-Hoon
Heo Dong-Hwa
Hwang Gyo-Jun
Kang Suk-Hyung
Kweon Hae-Yong
Jo You-Young
Kang Seok-Woo
Lee Kwang-Gill
Park Kye-Won
Han Kyu-Hyung
Park Jin-Seu
Eum Won-Sik
Cho Yong-Jun
Choi Hyun-Chul
Choi Soo-Young
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Abstract
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We investigated whether silk fibroin peptide derived from the silkworm, Bombyx mori, could inhibit inflammation and enhance the anti-inflammatory activity of Tat-superoxide dismutase (Tat-SOD), which was previously reported to effectively penetrate various cells and tissues and exert anti-oxidative activity in a mouse model of inflammation. Inflammation was induced by topical treatment of mouse ears with 12-O-tetradecanoylphorbol-13-acetate (TPA). Histological, Western blot, and reverse transcription-polymerase chain reaction data demonstrated that silk fibroin peptide or Tat-SOD alone could suppress elevated levels of cyclooxygenase-2, interleukin-6, interleukin-1beta, and tumor necrosis factor-alpha induced by TPA. Moreover, silk fibroin peptide significantly enhanced the anti-inflammatory activity of Tat-SOD, although it had no influence on in vitro and in vivo transduction of Tat-SOD. Silk fibroin peptide exhibited anti- inflammatory activity in a mice model of inflammation. Therefore, silk fibroin peptide alone or in combination with Tat-SOD might be used as a therapeutic agent for various inflammatory diseases.
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KEYWORD
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Inflammation, Protein therapy, Silk fibroin, Tat-superoxide dismutase, TPA
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